ABSTRACT The objective of this study was to determine the effects that breeder age has on digestive and immune system development; the transfer of immunoglobulins to egg yolk, yolk sac, and neonate chicks; and the immune response of chicks up to 35 days old. Three ages (32, 42, and 52 weeks) of Hubbard breeders were studied with ages as treatments. A total of 425 eggs were weighed for each of the three treatments and incubated. After hatching, a total of 300 1-day-old chicks were used in each treatment. We studied the development of the gastrointestinal tract and immune system of progeny and IgY transfer from breeder to progeny. Chicks from 52-week-old breeders had greater gastrointestinal tract growth up to seven days of life and greater body weight at 14 days. Older breeders (52 weeks) had higher amounts of IgY in serum and egg yolk. Chicks from the youngest breeders (32-weeks-old) had a better immune response at two weeks post-vaccination. It can be concluded that the older breeders have a greater capacity to immunize progeny up to 14 days. Strategies can be developed to increase IgY in the serum of young breeders and, consequently, increase the innate immunity of the newly-hatched chicks.

RESUMO: Teve-se como objetivos neste estudo avaliar o efeito dos níveis de metionina + cistina (Met+Cis) digestível na dieta sobre os parâmetros hematológicos e bioquímicos séricos de frangos de corte na fase inicial e de crescimento. Para isso, utilizou-se 1.800 pintos, machos, da linhagem Coob 500, sendo 900 pintos para a fase inicial (1 a 21 dias de idade) e mais 900 pintos para a fase de crescimento (22 a 42 dias de idade), distribuídos em delineamento inteiramente casualizado composto de cinco tratamentos, com seis repetições de 30 aves. Os tratamentos consistiram em 0,545; 0,616; 0,711; 0,782 e 0,853% e 0,514; 0,571; 0,647; 0,704 e 0,761% de Met+Cis digestíveis para a fase inicial e de crescimento, respectivamente. Os resultados demonstraram que níveis de Met+Cis digestível na alimentação de frango de corte, acarretam alterações em parâmetros hematológicos como: hemácia, hematócrito, hemoglobina, leucócitos totais, relação heterófilo: linfócito; e bioquímico sérico como: ácido úrico, albumina, colesterol total, proteínas séricas totais (PST), lipoproteína de baixa densidade (LDL) e triglicerídeos (TG). Os níveis digestíveis de Met + Cys na dieta de frangos de corte afetam os parâmetros hematológicos e a bioquímica sérica, principalmente em níveis mais elevados. A partir do nível de inclusão 0.761 de Met + Cist na dieta de frangos de corte, começam a aparecer alterações nos glóbulos vermelhos, hemoglobina e hematócrito.

ABSTRACT: The objective of this study was to evaluate the effect of digestible methionine and cystine (Met + Cys) levels on the hematological and serum biochemical parameters of broiler chickens during the initial and growth stages. For this, 1,800 male chicks of the Coob 500 strain were used, with 900 chicks in the initial phase (1 to 21 days old) and 900 chicks in the growth phase (22 to 42 days old), distributed in a completely randomized design of five treatments with six replicates of 30 birds. The treatments consisted of 0.545, 0.616, 0.711, 0.782, and 0.853%; and 0.514, 0.571, 0.647, 0.704, and 0.761% digestible Met + Cys for “1 to 21” and “22 to 42” days of breeding, respectively. Results showed that digestible Met + Cys levels in broiler feed altered some hematological parameters (erythrocyte, hematocrit hemoglobin, total leukocytes, heterophile: lymphocyte) and serum biochemistry (uric acid, PST, total LDL, and TG). The digestible Met + Cys levels in the diet of broilers affected the hematological parameters and serum biochemistry, especially at higher levels. From the inclusion level 0.761 of Met + Cist in the broiler diet, red blood cells, hemoglobin and hematocrit changes begin to appear.

The objective of this study to verify the physicochemical quality of commercial washed and unwashed eggs, experimentally inoculated on the shell with Pseudomonas aeruginosa and stored at 5 and 25 ºC for 30 days. A total of 384 eggs, classified as large, from light Dekalb White laying hens at 30 to 40 weeks of age, were used. The experimental design consisted of two blocks in a 2 × 2 × 2 factorial arrangement (contamination, washing, and refrigeration) with six replicates. The sanitization was performed by mechanical washing (hot water with chlorhexidine 20% and 8% active content). Eggs were contaminated by handling with 1.5 × 10(5) colony-forming units (cfu) of Pseudomonas aeruginosa/mL solution, and stored at 5 and 25 ºC for 30 days. Each ten days, analyses of the eggs were carried out, for the assessment of physical (egg weight, specific gravity, shell thickness, yolk, albumen and shell percentage, Haugh unit, yolk and albumen rates) and chemical (albumen and yolk pH) characteristics. There were interactions between sanitization, storage temperature and contamination. The cooling process maintained the egg internal quality even when there was contamination on the shell by Pseudomonas aeruginosa inoculum. Cooling slows down the weight loss and promotes better internal physical and chemical quality of the eggs during the 30 days of storage regardless of the contamination and washing processes.

Avaliou-se o perfil bioquímico do soro de frangos de corte alimentados com a enzima α-amilase produzida por dois microrganismos. Produziram-se dois extratos, um com a-amilase obtida a partir de Cryptococcus flavus em meio de levedura comercial e outro com Aspergillus niger HM2003 em meio de proteína de soja e amido comercial, com atividade de 9,58 U/mL e 10,0 U/mL, respectivamente. Utilizaram-se 360 pintos de corte Cobb 500 de 1 dia de idade e com 49,72 ± 0,68 g de peso vivo inicial. As aves foram alojadas em baterias e foram criadas até os 21 dias de idade. Foram utilizados três dietas, cada uma com cinco repetições de 12 aves, em delineamento inteiramente casualizado. A primeira dieta (basal) foi formulada sem adição de enzima e as outras duas receberam a suplementação de a-amilase produzida por cultivo de Cryptococcus flavus e Aspergillus niger HM2003. Dietas à base de milho e soja foram formuladas em duas fases: pré-inicial (1-7 dias) e inicial (8-21 dias). Na fase pré-inicial, foram observados os seguintes valores médios para cálcio (6,90 e 5,99 mg/dL), proteína plasmática (2,0 e 2,50 g/dL) e fosfatase alcalina (979,98 e 974,66 UI/L), respectivamente para Cryptococcus flavus e Aspergillus niger HM2003. A dieta acrescida de a-amilase obtida a partir de Aspergillus niger HM2003 determinou maior concentração sérica de fósforo. Na fase inicial, os resultados significativos relacionaram-se a potássio quando avaliadas dietas com adição de a-amilase pelas duas fontes. A incorporação das enzimas testadas não proporciona alterações metabólicas ou toxicidade nos animais.

It was evaluated the biochemical serum profile of broilers fed rations supplemented with α-amylase produced by two microorganisms. Two extracts were produced, one was produced with a-amylase obtained from Cryptococcus flavus in a commercial yeast-based medium and the other with Aspergillus niger HM2003 produced in soybean protein and commercial starch medium with activity of 9.58 U/mL and 10.0 U/mL, respectively. A total of 360 Cobb 500 male 1-d broiler chicks with 49.72 ± 0.68 g of initial body weight were used in the experiment. Birds were housed in heated batteries from one to 21 days of age. It was used three diets, each one with five replicates of 12 birds in a complete random design. The first diet (basal) was formulated without the addition of enzyme and the other two were supplemented with a-amylase produced by Cryptococcus flavus and Aspergillus niger HM2003 cultivation. Corn and soybean based diets were formulated in two phases: pre-starter phase (from 1 to 7 days of age) and starter phase (from 8 to 21 days). In pre-starter phase, it was observed the following average values for calcium (6.90 and 5.99 mg/dL), plasma protein (2.0 and 2.5 g/dL) and alkaline phosphatase activity (979.98 e 974.66 UI/L) for Criptococcus flavus and Aspergillus niger HM2003, respectively. Diet added with a-amylase obtained from Aspergillus niger HM2003 increased phosphorus serum concentration. In the starter phase, significative results were related to potassium when diets added with a-amylase were evaluated by the two sources. Incorporation of tested enzymes does not provide metabolic changes neither toxicity in the animals.